Hampton代理，Hampton上海代理，Hampton北京代理，Hampton廣州代理，Hampton總代理，Hampton一級代理，Hampton特約代理，Hampton現(xiàn)貨上海金畔生物科技有限公司Hampton專業(yè)代理
Hampton蛋白晶體接種Seeding Tool蛋白質(zhì)晶體生長方法
Seeding Tool |
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Applications
Features
DescriptionDuring streak seeding, one touches the Seeding Tool to crystalline material to dislodge, remove and transfer small crystals (seeds) to a drop that will support the growth of potentially larger and more perfect crystals. A seed can provide a template on which additional macromolecules can assemble and under the proper conditions, grow to form a large single crystal. Using seeding can avoid problems associated with growing crystals from spontaneous nucleation. Seeds can grow into larger crystals in the metastable region of the solubility curve, which is a region of lower, relative supersaturation. One can also streak seed from phase separation or amorphous material as a diagnostic to confirm whether the material is crystalline. The Seeding Tool is a 1 cm natural fiber attached to a stainless steel pin and plastic handle. The Seeding Tool is supplied with a cover to protect the fiber and when the cover is placed on the back side of the Seeding Tool, it makes an excellent handle.
上海金畔生物科技有限公司服務熱線:18301939375 ??QQ;3259632176官網(wǎng)：http://www.jinpanbio.cn ?上海市浦東新區(qū)東靖路669弄38-101 ??郵編：201208 |
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上海金畔生物科技有限公司服務熱線:18301939375 ??QQ;3259632176官網(wǎng)：http://www.jinpanbio.cn ?上海市浦東新區(qū)東靖路669弄38-101 ??郵編：201208 |
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Applications
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Sitting drop crystallization | |
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Seeding | |
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Heavy atom soaks | |
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Cryo transfers | |
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Large drops to produce large crystals for neutron diffraction studies |
Features
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Siliconized, 9 well glass plates | |
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Concave wells | |
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Polystyrene box and support |
Materials
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Siliconized glass and polystyrene |
上海金畔生物科技有限公司
服務熱線:18301939375 ??QQ;3259632176
官網(wǎng)：http://www.jinpanbio.cn ?
上海市浦東新區(qū)東靖路669弄38-101 ??郵編：201208
Description
When setting up a Sandwich Box, one pipets 25 ml of crystallization reagent or dehydrant into the bottom of the Sandwich Box, then places a support into the box. The 9 well depression plate is placed upon the support. Drops of sample and reagent are pipetted into the depressions and the lid of the Sandwich Box is sealed closed with vacuum grease. Why Sandwich Boxes? They allow one to use very, very large drops. They are optically superior to plastic plates and glass slides, and offer different equilibration kinetics than other crystallization plates. Each of the Sandwich Box components can be washed and reused so there is little waste with the Sandwich Box Setup. These plates can be used during screening and optimization, but are best suited for final optimization and production of crystals. Since the glass plates can be removed from the boxes, crystal mounting is convenient. The siliconized 9 well depression plates are also useful for heavy atom soaks, cryo solution dilution and transfers, and seeding experiments where serial dilutions are involved. Sandwich Box Setups are available as a complete set or as individual components so one can customize the system to meet their needs. Square cover slides can be used to seal individual reservoirs but are not typically used in a crystallization setup. The Sandwich Box Setup consists of a square plastic box (4?5/16” x 4?5/16” x 1?1/8”), a polystyrene plate support, and a siliconized, glass plate (4” L x 3?3/8” W (100 mm x 85 mm)) with nine concave depressions (7/8” O.D. x 1/4” D (22 mm x 7 mm)).
Drop sizes up to 800 microliters are possible with the Sandwich Box Setup.
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| References | ||
| 1. | In situ X-ray crystallography. A. McPherson. J. Appl. Cryst. (2000). 33, 397-400. | |
| 2. | Preliminary joint X-ray and neutron protein crystallographic studies of endoxylanase II from the fungus Trichoderma longibrachiatum. Kovalevsky, A. Y., Hanson, B. L., Seaver, S., Fisher, S. Z., Mustyakimov, M. & Langan, P. (2011). Acta Cryst. F67, 283-286. | |
上海金畔生物科技有限公司
服務熱線:18301939375 ??QQ;3259632176
官網(wǎng)：http://www.jinpanbio.cn ?
上海市浦東新區(qū)東靖路669弄38-101 ??郵編：201208
? ? ? 美國Hampton Research公司位于加利福尼亞州，是一家專門從事蛋白質(zhì)晶體研究的生產(chǎn)商。
該公司向全球科研人員提供全面的晶體研究試劑和相關實驗耗材，同時提供晶體設計和合成方面的定制服務。Hampton Research公司以其先進的生物大分子結晶技術和廣泛實用的產(chǎn)品線，不僅為全世界晶體研究人員的工作帶來了便捷，而且也受益于科研人員的回饋不斷發(fā)展自己，目前已成為晶體研究領域最受信賴的品牌之一。
Hampton research主要產(chǎn)品：
Crystallization Screens 用于篩選和優(yōu)化蛋白質(zhì)/多肽/核酸結晶的一系列經(jīng)驗證的高精配方型溶液
Optimize Reagents 結晶級別鹽類，聚合物，有機溶液或緩沖液
Crystallization Plates, Hardware & Accessories 結晶板（懸滴法和坐滴法，微量透析，微配液，自由界面擴散，凝膠以及無容器懸浮法）及其他配件
Cryocrystallography 低溫晶體學耗材如Crystalcap system,cryoloops
Goniometer Heads&Supplies 測角器頭和配件
Protein Crystallization Standards 結晶級別標準蛋白
Custom Shop Crystallization Reagents 定制試劑盒和結晶溶液內(nèi)的單一組分
StockOptions Kits 內(nèi)含鹽類，緩沖液和冷凍液的簡便型試劑盒
Tools，Seeding&Resin 結晶用探針，結晶筆，seeding法結晶工具
Capillary Mounts & Supplies 毛細管,玻璃纖維, 蠟,黏合劑和密封劑
??在生命科學領域，康寧為業(yè)界提供了豐富的實驗室和生物醫(yī)藥生產(chǎn)研發(fā)的產(chǎn)品。慶祝上海金畔生物正式成為?Hampton蛋白結晶板（懸滴法和坐滴法）的簽約代理商，在這里要感謝廣大客戶多年來對上海金畔生物科技有限公司的支持和厚愛，我們將一如既往的為廣大客戶帶來Hampton蛋白結晶板（懸滴法和坐滴法）高品質(zhì)的產(chǎn)品和服務，歡迎廣大新老客戶來電咨詢。
如果您對上述產(chǎn)品感興趣，請致電18301939375或QQ：3259632176，上海金畔生物科技有限公司進一步了解詳情。 |